Question 565
{"accessible":false,"alternatives":[{"id":2831,"text":"GFAP"},{"id":2832,"text":"MAP2"},{"id":2833,"text":"NeuN"},{"id":2834,"text":"neurofilament"},{"id":2835,"text":"synaptophysin"}],"archived":false,"correctAlternativeId":2831,"explanation":"\u003cp\u003eGFAP (glial fibrillary acid protein) is a commonly used target for immunohistochemistry and is positive in many glial cells and tumors of glial origin. GFAP is the building block for intermediate filaments which are abundant in the cytoplasms particularly of astrocytes. It is not present in neurons.\u0026nbsp;\u003c/p\u003e\u003cp\u003eGFAP is only rarely expressed in neurons under certain specific circumstances (1)\u0026nbsp;but generally, it is considered an astrocytic/glial marker which does not stain neurons. \u0026nbsp;\u003c/p\u003e\u003cp\u003e1.\u0026nbsp;E M Hol, R F Roelofs, E Moraal, M A F Sonnemans, J A Sluijs, E A Proper, P N E de Graan, D F Fischer, F W van Leeuwen. Neuronal expression of GFAP in patients with Alzheimer pathology and identification of novel GFAP splice forms. Molecular Psychiatry. 8 (9): 786. \u0026lt;a href=\"https://doi.org/doi:10.1038/sj.mp.4001379\"\u0026gt;doi:doi:10.1038/sj.mp.4001379\u0026lt;/a\u0026gt; \u0026lt;span class=\"ref_v4\"\u0026gt;\u0026lt;/span\u0026gt;\u003c/p\u003e","id":565,"imageUrl":null,"imageAttribution":null,"imageAttributionCaseInfo":null,"firstQuestionPath":"/questions/565","nextQuestionPath":null,"relatedArticles":[{"id":51742,"title":"Glial fibrillary acid protein (GFAP)","link":"/articles/glial-fibrillary-acid-protein-gfap?lang=us"}],"alsoUsedIn":[],"stem":"\u003cp\u003eWhich of the following immunohistochemistry markers does\u0026nbsp;\u003cstrong\u003eNOT\u003c/strong\u003e\u0026nbsp;usually stain neurons?\u0026nbsp;\u003c/p\u003e","menuLinks":[{"text":"Report problem with question","url":"https://docs.google.com/forms/d/e/1FAIpQLSfO3soWYhOjJ7yErSysyCe5V4A1CqW7WK3rDA7MtAkecMGqNw/viewform?entry.1624461248\u0026entry.553583435=https://radiopaedia.org/questions/565"}],"attemptsPercentages":[{"alternativeId":"2831","percentage":44},{"alternativeId":"2835","percentage":8},{"alternativeId":"2832","percentage":34},{"alternativeId":"2833","percentage":2},{"alternativeId":"2834","percentage":12}],"promptToLogin":false,"questionManager":false,"articleId":"neuron"}