From the case:
Immunohistochemistry (diagram)
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In indirect immunohistochemistry, the first (primary antibody) binds to the desired antigen and the second one (secondary antibody) has the enzyme attached and it binds to the primary antibody. A substrate is then introduced that the enzyme converts to a colored product.
Often this is combined with a counter-stain for context (e.g. hematoxylin to stain cell nuclei blue).
Case Discussion
Diagram created by Frank Gaillard. Histology slide adapted from an ependymoma case (Frank Gaillard, rID: 28325).
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